Article
Changes in osteoblast phenotype during differentiation of enzymatically isolated rat calvaria cells.
Bone - 1 May 1998
Wada Y, Kataoka H, Yokose S, Ishizuya T, Miyazono K, Gao Y H, Shibasaki Y, Yamaguchi A
Abstract excerpt
Osteoblasts enzymatically isolated from newborn rat calvariae show various phenotypes including formation of mineralized bone nodules in culture. We investigated the temporal changes in osteoblast phenotype in these cells up to day 20 in culture. These cells formed unmineralized nodules by day 5. Mineralization was observed at the center of nodules by day 10, and nodules became larger on day 15. The nodules were...
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