Changes in osteoblast phenotype during differentiation of enzymatically isolated rat calvaria cells.

doi:10.1016/s8756-3282(98)00039-8

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Article

Changes in osteoblast phenotype during differentiation of enzymatically isolated rat calvaria cells.

Bone - 1 May 1998

Wada Y, Kataoka H, Yokose S, Ishizuya T, Miyazono K, Gao Y H, Shibasaki Y, Yamaguchi A

Abstract excerpt

Osteoblasts enzymatically isolated from newborn rat calvariae show various phenotypes including formation of mineralized bone nodules in culture. We investigated the temporal changes in osteoblast phenotype in these cells up to day 20 in culture. These cells formed unmineralized nodules by day 5....

Topics

Alkaline Phosphatase
Animals
Blotting, Northern
Bone Morphogenetic Protein 2
Bone Morphogenetic Protein 4
Bone Morphogenetic Proteins
Calcification, Physiologic
Cell Differentiation
Cell Separation
Cells, Cultured
Immunohistochemistry

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

In Situ Hybridization

Osteoblasts

Osteocalcin

Phenotype

RNA, Messenger

Rats

Receptor, Parathyroid Hormone, Type 1

Source

Open in PubMed

Identifiers

PMID: 9600781
DOI: 10.1016/s8756-3282(98)00039-8

Genes

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-9600781-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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