Article
Rat tibial osteoblasts III: propagation in vitro is accompanied by enhancement of osteoblast phenotype.
Bone - 1 Jul 1997
Manduca P, Palermo C, Caruso C, Brizzolara A, Sanguineti C, Filanti C, Zicca A
Abstract excerpt
Postproliferative confluent cultures of primary rat tibial osteoblasts (ROB), cultured in medium supplemented with ascorbic acid and beta-glycerophosphate (AS-bGP, differentiation medium) express, in sequence, specific bone markers which identify a succession of maturation stages, and eventually...
Topics
- Alkaline Phosphatase
- Animals
- Ascorbic Acid
- Blotting, Northern
- Calcification, Physiologic
- Calcium
- Cell Differentiation
- Cell Division
- Cells, Cultured
- Chemotaxis
- Cloning, Molecular
- Culture Media, Conditioned
- DNA
- Fluorometry
- Glycerophosphates
- Immunohistochemistry
- Integrin-Binding Sialoprotein
- Osteoblasts