Matrix mineralization and the differentiation of osteocyte-like cells in culture.

doi:10.1002/jbmr.5650100209

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Article

Matrix mineralization and the differentiation of osteocyte-like cells in culture.

Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research - 1 Feb 1995

Mikuni-Takagaki Y, Kakai Y, Satoyoshi M, Kawano E, Suzuki Y, Kawase T, Saito S

Abstract excerpt

Osteocyte-like cells were prepared by sequentially treating calvaria from newborn rats with collagenase and chelating agents. On a reconstituted gel of basement membrane components, cells from the third collagenase digest displayed a round shape and expressed the highest level of alkaline phospha...

Topics

5'-Nucleotidase
Alkaline Phosphatase
Animals
Animals, Newborn
Basement Membrane
Bone Matrix
Calcification, Physiologic
Casein Kinase II
Cell Differentiation
Cell Separation
Cells, Cultured
DNA-Binding Proteins
Dendrites
Edetic Acid
Frontal Bone
Osteoblasts
Osteocalcin
Osteocytes

Source

Open in PubMed

Identifiers

PMID: 7754802
DOI: 10.1002/jbmr.5650100209

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026. Prepared record publication-7754802-2026-08-30.1. Manifest publication-graph-cohort-2026-08-30.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 30 Aug 2026.

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