Article
Cloning-free PCR-based allele replacement methods.
Genome research - 1 Dec 1997
Erdeniz N, Mortensen U H, Rothstein R
Abstract excerpt
Efficient homologous recombination permits the directed introduction of specific mutations into the yeast genome. Here we describe a cloning-free, PCR-based allele replacement method that simplifies allele transfer between yeast strains. The desired allele from one strain is amplified by PCR, along with a selectable/counterselectable marker. After transformation, the resident allele in the target strain is...
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