Article
Manipulating yeast genome using plasmid vectors.
Methods in enzymology - 1 Jan 1990
Stearns T, Ma H, Botstein D
Abstract excerpt
The vectors and techniques described here enable one to manipulate the yeast genome to meet specific needs. Genes can be cloned, and the clone used to delete the wild-type gene from the chromosome, or replace it with mutant versions. Mutants derived by classical methods, such as mutagenesis of whole cells, or by reversion of a phenotype, can be cloned and analyzed in vitro. Yeast genes and foreign genes can...
Topics
- Cloning, Molecular
- Gene Expression
- Genes, Fungal
- Genetic Vectors
- Mutation
- Plasmids
- Recombination, Genetic
- Saccharomyces cerevisiae