Article
Methods in laboratory investigation. Rapid and simple detection of c-Ki-ras2 gene codon 12 mutations by nonradioisotopic single-strand conformation polymorphism analysis.
Laboratory investigation; a journal of technical methods and pathology - 1 Mar 1993
Sugano K, Kyogoku A, Fukayama N, Ohkura H, Shimosato Y, Sekiya T, Hayashi K
Abstract excerpt
BACKGROUND: To achieve simple and rapid detection of codon 12 mutations of the c-Ki-ras2 gene, we developed a nonradioisotopic single-strand conformation polymorphism analysis using silver stain. EXPERIMENTAL DESIGN: After a conventional polymerase chain reaction, amplified DNA fragments were mix...
Topics
- Alleles
- Codon
- Colorectal Neoplasms
- DNA, Single-Stranded
- Electrophoresis, Polyacrylamide Gel
- Fibroblasts
- Humans
- Immunoblotting
- Lung Neoplasms
- Mutation
- Pancreatic Neoplasms
- Polymerase Chain Reaction
- Polymorphism, Genetic
- Proto-Oncogene Proteins p21(ras)