Effects of multiple mutations at the conserved TATA sequence of bacteriophage SP6 promoter on transcription efficiency.

Abstract excerpt

Mutations of A<==>T were introduced individually and multiple to TATA from -4 to -1 of the phage SP6 promoter and their effects on transcription initiation efficiency measured in vitro. All 15 mutants tested were less active than the wild type. Mutation at -4T nearly abolishes promoter activity independent of other changes, and alteration at -3A reduces promoter activity substantially. On the other hand, effects...

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