Effects of multiple mutations at the conserved TATA sequence of bacteriophage SP6 promoter on transcription efficiency.

Article

Effects of multiple mutations at the conserved TATA sequence of bacteriophage SP6 promoter on transcription efficiency.

Biochemistry and molecular biology international - 1 Sept 1993

Kim S S, Hong Y, Kang C

Abstract excerpt

Mutations of A<==>T were introduced individually and multiple to TATA from -4 to -1 of the phage SP6 promoter and their effects on transcription initiation efficiency measured in vitro. All 15 mutants tested were less active than the wild type. Mutation at -4T nearly abolishes promoter activity i...

Topics

Bacteriophages
Base Sequence
DNA-Directed RNA Polymerases
Molecular Sequence Data
Mutation
Promoter Regions, Genetic
TATA Box
Transcription, Genetic

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.