Article
N-terminal DNA-binding domains contribute to differential DNA-binding specificities of NF-kappa B p50 and p65.
Molecular and cellular biology - 1 Feb 1993
Toledano M B, Ghosh D, Trinh F, Leonard W J
Abstract excerpt
We previously reported that either oxidation or alkylation of NF-kappa B in vitro abrogates DNA binding. We used this phenomenon to help elucidate structural determinants of NF-kappa B binding. We now demonstrate that Cys-62 of NF-kappa B p50 mediates the redox effect and lies within an N-termina...
Topics
- Amino Acid Sequence
- Base Sequence
- Binding Sites
- Cloning, Molecular
- Cysteine
- DNA
- Humans
- Molecular Sequence Data
- Mutation
- NF-kappa B
- Oxidation-Reduction
- Sequence Homology, Amino Acid