Article
Proteolysis of Saccharomyces cerevisiae RNase H1 in E coli.
Biochimie - 1 Jan 1993
Cerritelli S M, Shin D Y, Chen H C, Gonzales M, Crouch R J
Abstract excerpt
Expression of S cerevisiae RNase H1 in E coli leads to the formation of a proteolytic product with a molecular mass of 30 kDa that is derived from the 39-kDa full length protein. The 30-kDa form retains RNase H1 activity, as determined by renaturation gel assay. The amount of proteolysis observed depends on the procedure used in preparing the cell extracts for protein analysis. The cleavage site on the amino acid...
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