Article
Selective cloning of genes encoding RNase H from Salmonella typhimurium, Saccharomyces cerevisiae and Escherichia coli rnh mutant.
Molecular & general genetics : MGG - 1 Jul 1991
Itaya M, McKelvin D, Chatterjie S K, Crouch R J
Abstract excerpt
We have cloned genes encoding RNase H from Escherichia coli rnh mutants, Salmonella typhimurium and Saccharomyces cerevisiae. Selection was accomplished by suppression of the temperature-sensitive growth phenotype of Escherichia coli strains containing the rnh-339::cat and either recB270 (Ts) or recC271 (Ts) mutations. RNases H from E. coli and S. typhimurium contained 155 amino acid residues and differed at only...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.
