Article
Selective cloning of genes encoding RNase H from Salmonella typhimurium, Saccharomyces cerevisiae and Escherichia coli rnh mutant.
Molecular & general genetics : MGG - 1 Jul 1991
Itaya M, McKelvin D, Chatterjie S K, Crouch R J
Abstract excerpt
We have cloned genes encoding RNase H from Escherichia coli rnh mutants, Salmonella typhimurium and Saccharomyces cerevisiae. Selection was accomplished by suppression of the temperature-sensitive growth phenotype of Escherichia coli strains containing the rnh-339::cat and either recB270 (Ts) or...
Topics
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Endoribonucleases
- Escherichia coli
- Genes, Bacterial
- Genes, Fungal
- Molecular Sequence Data
- Mutation
- Nucleic Acid Hybridization
- Phenotype
- Plasmids
- Promoter Regions, Genetic
- Ribonuclease H
- Saccharomyces cerevisiae
- Salmonella typhimurium
- Sequence Homology, Nucleic Acid