Article
Differential stability of human interleukin 1 beta fragments expressed in yeast.
Protein engineering - 1 Jan 2000
Baldari C, Massone A, Macchia G, Telford J L
Abstract excerpt
We have made deletions in a human interleukin 1 beta cDNA cloned in a yeast vector capable of directing the expression and secretion of foreign protein. Deletions at either the 5' or 3' end of the cDNA result in a dramatic drop in yield of protein. An investigation of some possible causes points to instability of the truncated peptides as a major factor contributing to low yield. The results suggest that correct...
Topics
- Base Sequence
- Chromosome Deletion
- Gene Expression Regulation
- Genetic Vectors
- Interleukin-1
- Mutation
- Nucleic Acid Hybridization
- RNA
- Saccharomyces cerevisiae