Mutational analysis of K28 preprotoxin processing in the yeast Saccharomyces cerevisiae.

doi:10.1099/00221287-148-5-1317

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Article

Mutational analysis of K28 preprotoxin processing in the yeast Saccharomyces cerevisiae.

Microbiology (Reading, England) - 1 May 2002

Riffer Frank, Eisfeld Katrin, Breinig Frank, Schmitt Manfred J

Abstract excerpt

K28 killer strains of Saccharomyces cerevisiae are permanently infected with a cytoplasmic persisting dsRNA virus encoding a secreted alpha/beta heterodimeric protein toxin that kills sensitive cells by cell-cycle arrest and inhibition of DNA synthesis. In vivo processing of the 345 aa toxin precursor (preprotoxin; pptox) involves multiple internal and carboxy-terminal cleavage events by the prohormone...

Topics

Disulfides
Endoplasmic Reticulum
Gene Deletion
Killer Factors, Yeast
Membrane Proteins
Models, Biological
Mutagenesis, Site-Directed
Mycotoxins
Oligopeptides
Phenotype
Proprotein Convertases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Protein Processing, Post-Translational

Protein Sorting Signals

Protein Transport

Saccharomyces cerevisiae

Saccharomyces cerevisiae Proteins

Serine Endopeptidases

Subtilisins

Source

Open in PubMed

Identifiers

PMID: 11988505
DOI: 10.1099/00221287-148-5-1317

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