Article
Characterization of a vacuolar protease in Neurospora crassa and the use of gene RIPing to generate protease-deficient strains.
The Journal of biological chemistry - 6 Sept 1996
Vázquez-Laslop N, Tenney K, Bowman B J
Abstract excerpt
We have isolated a gene from Neurospora crassa that appears to encode a pepstatin-sensitive protease found both in membranes and in soluble contents of vacuoles. The gene contains two introns and encodes a 396-residue protein with a molecular mass of 42,900 Da. Because of the similarity of the protein to proteinase A in Saccharomyces cerevisiae the gene has been named pep-4. Strains with mutations in the pep-4...
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