Article
Allelic discrimination by nick-translation PCR with fluorogenic probes.
Nucleic acids research - 11 Aug 1993
Lee L G, Connell C R, Bloch W
Abstract excerpt
Nick-translation PCR was performed with fluorogenic probes. Two probes were used: one complementary to a sequence containing the F508 codon of the normal human cystic fibrosis (CF) gene (wt DNA) and one complementary to a sequence containing the delta F508 three base pair deletion (mut DNA). Each probe contained a unique and spectrally resolvable fluorescent indicator dye at the 5' end and a common quencher dye...
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