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Article

Rapid F508del and F508C assay using fluorescent hybridization probes.

Genetic testing - 1 Jan 1999

Gundry C N, Bernard P S, Herrmann M G, Reed G H, Wittwer C T

Abstract excerpt

Amplification and fluorescent genotyping of the cystic fibrosis F508del locus was achieved from human genomic DNA in less than 30 min. The hybridization of adjacent fluorescent probes at the mutation site was monitored by resonance energy transfer between fluorescein and Cy5 during heating or cooling. Characteristic curves were obtained for each genotype; the first derivative of these fluorescent curves has a...

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Identifiers

PMID: 10627945
DOI: 10.1089/gte.1999.3.365

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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