Article
Perturbation of the carboxy terminus of HIV-1 Rev affects multimerization on the Rev responsive element.
Biochemistry - 31 Aug 1993
Daly T J, Rennert P, Lynch P, Barry J K, Dundas M, Rusche J R, Doten R C, Auer M, Farrington G K
Abstract excerpt
Perturbations within the transactivation and carboxy-terminal domains of HIV-1 Rev were examined for effects on Rev responsive element (RRE) binding activities in vitro and biological activity in vivo. Binding affinities, specificities, and multimerization of the transactivation mutants M10 and R...
Topics
- Amino Acid Sequence
- Cells, Cultured
- Cyanogen Bromide
- DNA, Viral
- Gene Products, rev
- HIV-1
- Molecular Sequence Data
- Mutation
- RNA, Viral
- Sequence Deletion
- Transcriptional Activation
- Transfection
- rev Gene Products, Human Immunodeficiency Virus