Real-time kinetics of HIV-1 Rev-Rev response element interactions. Definition of minimal binding sites on RNA and protein and stoichiometric analysis.

Abstract excerpt

The kinetics of interaction between the human immunodeficiency virus-1 Rev protein and its RNA target, Rev response element (RRE) RNA was determined in vitro using a biosensor technique. Our results showed that the primary Rev binding site is a core stem-loop RNA molecule of 30 nucleotides that bound Rev at a 1:1 ratio, whereas the 244-nucleotide full-length RRE bound four Rev monomers. At high Rev...

Topics

• Amino Acid Sequence
• Base Sequence
• Binding Sites
• Binding, Competitive
• Gene Products, rev
• Genes, env
• HIV-1
• Kinetics
• Molecular Sequence Data
• Mutation
• Nucleic Acid Conformation
• Oligoribonucleotides
• Peptide Fragments
• Protein Binding
• RNA-Binding Proteins
• Surface Plasmon Resonance
• rev Gene Products, Human Immunodeficiency Virus