Article
Simplified isoelectric focusing/immunoblotting determination of apoprotein E phenotype.
Clinical chemistry - 1 Jan 1994
Kataoka S, Paidi M, Howard B V
Abstract excerpt
We developed a rapid, accurate method for phenotyping apoprotein E that can be used for large-scale population studies. In this method, adapted from the method of Kamboh et al. (J Lipid Res 1988;29:1535-43), 10-microL plasma samples are incubated with dithiothreitol and Tween-20 for 15 min and then applied to 5% polyacrylamide gels containing ampholyte (pH 4.5-8) and urea (3 mol/L). After 2 h of isoelectric...
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