Article
Improved phenotyping of apolipoprotein E: application to population frequency distribution.
Clinical chemistry - 1 Nov 1990
Hill J S, Pritchard P H
Abstract excerpt
A simple procedure for phenotyping apolipoprotein (apo) E directly from plasma has been developed for use in the lipid clinic laboratory. In this new method, 10 microL of serum or plasma is pretreated with neuraminidase (EC 3.2.1.18), which removes the sialic acid residues from apo E and eliminates additional bands, thereby ensuring correct phenotype assignment. After a rapid delipidation step, the samples are...
Topics
- Alleles
- Apolipoproteins E
- British Columbia
- Gene Frequency
- Humans
- Isoelectric Focusing
- Neuraminidase
- Phenotype