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[Isoelectric focusing and immunoblotting identification of human ApoE phenotype].

Hua xi yi ke da xue xue bao = Journal of West China University of Medical Sciences = Huaxi yike daxue xuebao - 1 Mar 1998

Zhang Z, Fan P, Liu B

Abstract excerpt

A simple, rapid and accurate method for phenotyping sera apoE has been developed. In this method 10 microliters serum or plasma are incubated with Dithiothreitol and Tween-20 for 15 min and then applied to 5% polyacrylamid gel containing pH 4-8 ampholyte and 3 mol/L urea. After 2 h focusing, the apoE bands are transferred to Nitrocellulose membrane. apoE bands are made visible by immunoblotting and TMB is used as...

Topics

Apolipoproteins E
Blotting, Western
Female
Humans
Isoelectric Focusing
Male
Phenotype
Polymorphism, Genetic

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 10683994

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