Article
Rapid determination of apolipoprotein E phenotypes from whole plasma by automated isoelectric focusing using PhastSystem and immunofixation.
Journal of lipid research - 1 Jan 1994
Hackler R, Schäfer J R, Motzny S, Brand S, Kleine T O, Kaffarnik H, Steinmetz A
Abstract excerpt
The influence of the genetic apolipoprotein (apo) E isoforms on human plasma lipoproteins is well established. There is, however, still a need for a phenotyping procedure applicable in laboratories not specialized in lipid research. To this end, we developed a rapid, automated electrophoresis method for apoE phenotyping. Either self-made or commercially available precasted gels can be used. Fresh or frozen...
Topics
- Apolipoproteins E
- Blood Protein Electrophoresis
- Evaluation Studies as Topic
- Humans
- Isoelectric Focusing
- Phenotype
- Reproducibility of Results
- Sensitivity and Specificity