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Article

Apolipoprotein E phenotyping by isoelectric focusing in immobilized pH gradients and silver staining.

Electrophoresis - 1 Apr 1992

Cartier R, Sassolas A

Abstract excerpt

A simple and high resolution procedure of apoprotein E (apo E) phenotyping by isoelectric focusing with immobilized pH gradients and silver staining is described. This method needs delipidated very low density lipoproteins (isolated from 1 mL of serum) but obviates immunoblotting as well as neuraminidase treatment in routine applications because the sialylated forms are clearly separated. Immunoblotting (with...

Topics

Acrylamides
Alleles
Apolipoproteins E
Cysteamine
Genetic Variation
Hydrogen-Ion Concentration
Immunoblotting
Isoelectric Focusing
Neuraminidase
Phenotype
Silver Staining

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Source

Identifiers

PMID: 1628606
DOI: 10.1002/elps.1150130151

Genes

SAA1
SAA2

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1628606-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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