Article
Rapid and simple method for the identification of apolipoprotein E isomorphic phenotypes from whole serum.
Electrophoresis - 1 Apr 1992
Kohlmeier M, Drossel H J, Sinha P, Köttgen E
Abstract excerpt
For the identification of apolipoprotein E isomorphic phenotypes, fresh or thawed serum was analyzed without prior delipidation or other pretreatment. Using 5% polyacrylamide gels with a 40 mm interelectrode distance, the isoforms were separated by isoelectric focusing in immobilized pH gradients...
Topics
- Antibodies, Monoclonal
- Apolipoproteins E
- Humans
- Hydrogen-Ion Concentration
- Immunoblotting
- Isoelectric Focusing
- Phenotype
- Staining and Labeling
- Time Factors