Apolipoprotein E phenotyping from plasma by isoelectric focusing and immunoblotting.

doi:10.1620/tjem.160.301

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Article

Apolipoprotein E phenotyping from plasma by isoelectric focusing and immunoblotting.

The Tohoku journal of experimental medicine - 1 Apr 1990

Eto M, Watanabe K, Moriyama T, Makino I

Abstract excerpt

A method for apolipoprotein (apo) E phenotyping directly from plasma by isoelectric focusing (IEF) and immunoblotting was confirmed. Ten microliters plasma were delipidated. IEF in 5% polyacrylamide flat gel with 6.4 mol/l urea and 2.8% pharmalyte (PH 4-6.5) was carried out at 3,000 V for 1 hr. S...

Topics

Apolipoproteins E
Humans
Immunoblotting
Isoelectric Focusing
Phenotype

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