Article
Expression in yeast of three allelic cDNAs coding for human liver P-450 3A4. Different stabilities, binding properties and catalytic activities of the yeast-produced enzymes.
European journal of biochemistry - 1 Dec 1993
Peyronneau M A, Renaud J P, Jaouen M, Urban P, Cullin C, Pompon D, Mansuy D
Abstract excerpt
Three natural allelic cDNAs coding for P-450 3A4, the major form in human liver, namely NF25, NF10 and hPCN1, have been expressed in Saccharomyces cerevisiae. NF25 and hPCN1 were functionally expressed in yeast microsomes, yielding proteins with an absorption maximum at 448 nm in the CO-reduced d...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Catalysis
- Cloning, Molecular
- Cytochrome P-450 CYP3A
- Cytochrome P-450 Enzyme System
- DNA, Complementary
- Enzyme Stability
- Humans
- Liver
- Mixed Function Oxygenases
- Molecular Sequence Data
- Saccharomyces cerevisiae