Article
Heterologous expression of the allelic variant mu-class glutathione transferases mu and psi.
The Biochemical journal - 1 Jun 1991
Widersten M, Pearson W R, Engström A, Mannervik B
Abstract excerpt
cDNA encoding the more acidic form, glutathione transferase (GST) psi, of the polymorphic Mu-class GSTs discovered in liver, was mutated in the 5'-end to create an NcoI site, facilitating cloning into the expression plasmid pKK233-2. The protein expressed from this construct has a point mutation Pro-2----Ala-2, but gives a catalytically functional protein. Back-mutation of the codon for amino acid residue 2 gave...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- Cloning, Molecular
- Escherichia coli
- Gene Expression
- Gene Library
- Genetic Variation
- Glutathione Transferase
- Humans
- Isoenzymes