Separation of mutant and wild-type ribosomes based on differences in their anti Shine-Dalgarno sequence.

doi:10.1093/nar/21.23.5398

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Article

Separation of mutant and wild-type ribosomes based on differences in their anti Shine-Dalgarno sequence.

Nucleic acids research - 25 Nov 1993

Poot R A, Brink M F, Pleij C W, de Boer H A, van Duin J

Abstract excerpt

We describe a system to isolate 30S ribosomal subunits which contain targeted mutations in their 16S rRNA. The mutations of interest should be present in so-called specialized 30S subunits which have an anti-Shine-Dalgarno sequence that is altered from 5' ACCUCC to 5' ACACAC. These plasmid-encode...

Topics

Base Sequence
Cell Fractionation
Chromatography, Affinity
Escherichia coli
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Peptide Chain Initiation, Translational
Protein Biosynthesis
RNA, Ribosomal, 16S
Ribosomes

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