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Article

A single base change at 726 in 16S rRNA radically alters the pattern of proteins synthesized in vivo.

The EMBO journal - 1 Jan 1990

Prescott C D, Dahlberg A E

Abstract excerpt

A single base change in 16S rRNA (C-726 to G) was constructed by site-directed mutagenesis and cloned into the multicopy plasmid pKK3535 (generating pKK726G) which contains the complete rrnB operon from Escherichia coli. The mutant 16S rRNA was found predominantly in the 30S subunit fraction but was present in the 70S ribosomes. Protein analyses of the free 30S subunits revealed a decrease in the levels of...

Topics

Base Sequence
Cloning, Molecular
Electrophoresis, Gel, Two-Dimensional
Escherichia coli
Heat-Shock Proteins
Molecular Sequence Data
Mutation
Nucleic Acid Conformation
Operon
Plasmids
Protein Biosynthesis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

RNA, Ribosomal

RNA, Ribosomal, 16S

Ribosomes

Temperature

Transformation, Bacterial

Source

Open in PubMed Open full text

Identifiers

PMID: 2403929
DOI: 10.1002/j.1460-2075.1990.tb08107.x
PMCID: PMC551661

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2403929-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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