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Article

Ribosome engineering reveals the importance of 5S rRNA autonomy for ribosome assembly.

Nature communications - 9 Jun 2020

Huang Shijie, Aleksashin Nikolay A, Loveland Anna B, Klepacki Dorota, Reier Kaspar, Kefi Amira, Szal Teresa, Remme Jaanus, Jaeger Luc, Vázquez-Laslop Nora, Korostelev Andrei A, Mankin Alexander S

Abstract excerpt

5S rRNA is an indispensable component of cytoplasmic ribosomes in all species. The functions of 5S rRNA and the reasons for its evolutionary preservation as an independent molecule remain unclear. Here we used ribosome engineering to investigate whether 5S rRNA autonomy is critical for ribosome function and cell survival. By linking circularly permutated 5S rRNA with 23S rRNA we generated a bacterial strain...

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Identifiers

PMID: 32518240
DOI: 10.1038/s41467-020-16694-8
PMCID: PMC7283268

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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