Single base alterations upstream of the E. coli 16S rRNA coding region result in temperature-sensitive 16S rRNA expression.

doi:10.1016/0167-4781(90)90189-9

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Article

Single base alterations upstream of the E. coli 16S rRNA coding region result in temperature-sensitive 16S rRNA expression.

Biochimica et biophysica acta - 27 Aug 1990

Mori H, Dammel C, Becker E, Triman K, Noller H F

Abstract excerpt

We have isolated three new temperature-sensitive mutants of 16S rRNA, using the U1192 spectinomycin resistance as a selectable marker. These differ from our previously characterized ts mutants in that they map in the upstream leader region of the rRNA precursor (at positions -13, -30 and -59). Th...

Topics

Base Sequence
Escherichia coli
Genes, Bacterial
Genes, Suppressor
Molecular Sequence Data
Mutation
Operon
Phenotype
Plasmids
Polyribosomes
RNA, Ribosomal, 16S
Ribosomes
Spectinomycin
Temperature

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