Article
Tagging ribosomal protein S7 allows rapid identification of mutants defective in assembly and function of 30 S subunits.
Journal of molecular biology - 5 May 2000
Fredrick K, Dunny G M, Noller H F
Abstract excerpt
Ribosomal protein S7 nucleates folding of the 16 S rRNA 3' major domain, which ultimately forms the head of the 30 S ribosomal subunit. Recent crystal structures indicate that S7 lies on the interface side of the 30 S subunit, near the tRNA binding sites of the ribosome. To map the functional surface of S7, we have tagged the protein with a Protein Kinase A recognition site and engineered alanine substitutions...
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