Article
Allele-specific structure probing of plasmid-derived 16S ribosomal RNA from Escherichia coli.
Gene - 15 Jan 1993
Powers T, Noller H F
Abstract excerpt
Biochemical analysis of Escherichia coli ribosomes containing mutant 16S or 23S (r)ribosomal RNAs, produced via cloned rDNA genes on multicopy plasmids, has been hindered by the background of wild-type (wt) ribosomes containing host-derived rRNA. Here, we describe a method for the construction of unique priming sites in 16S rRNA that allow allele-specific structure probing of ribosomes containing plasmid-encoded...
Topics
- Alleles
- Base Sequence
- DNA, Bacterial
- DNA, Ribosomal
- DNA, Single-Stranded
- Escherichia coli
- Molecular Sequence Data
- Nucleic Acid Conformation
- Plasmids
- RNA, Bacterial
- RNA, Ribosomal, 16S