Article
A complex that contains proteins binding to the PSE and TATA sites in a human U6 small nuclear RNA promoter.
Gene - 21 Oct 1994
Goomer R S, Urso O, Kunkel G R
Abstract excerpt
The proximal promoter of a human U6 small nuclear RNA (snRNA)-encoding gene contains two separate elements, the proximal sequence element (PSE) and the TATA box. We investigated the interaction of the PSE- and TATA-binding proteins (PBP and TBP) with normal and mutant U6 proximal promoters using an electrophoretic mobility shift assay. We detected a complex containing both PBP and TBP bound to the wild-type U6...
Topics
- Base Sequence
- DNA Primers
- DNA-Binding Proteins
- Humans
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- RNA, Small Nuclear
- TATA Box
- TATA-Box Binding Protein
- Transcription Factors