Article
Activity of the E75E76 mutant of the alpha subunit of casein kinase II from Xenopus laevis.
FEBS letters - 14 Feb 1994
Gatica M, Jedlicki A, Allende C C, Allende J E
Abstract excerpt
The cDNA gene coding for the alpha subunit of Xenopus laevis casein kinase II was mutated using the overlap extension PCR method. The mutation substituted glutamic acids for Lys75 and Lys76, changing the charge distribution of a very basic sequence found in the alpha subunit. Expression of the mutated cDNA in a pT7-7 vector in E. coli yielded an active mutant recombinant protein that was extensively purified....
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