Article
Activation of human factor V by meizothrombin.
The Journal of biological chemistry - 10 Jun 1994
Tans G, Nicolaes G A, Thomassen M C, Hemker H C, van Zonneveld A J, Pannekoek H, Rosing J
Abstract excerpt
A recombinant human prothrombin was prepared in which Arg155 was replaced by Ala. The recombinant prothrombin was converted into a meizothrombin derivative (R155A meizothrombin) that was resistant to autocatalytic removal of the fragment 1 domain. R155A meizothrombin appeared to be a potent factor V activator in reaction mixtures that contained negatively charged phospholipid vesicles. Factor V activation by...
Topics
- Base Sequence
- Enzyme Precursors
- Factor V
- Humans
- Kinetics
- Liposomes
- Molecular Sequence Data
- Mutation
- Phosphatidylcholines
- Phosphatidylserines
- Prothrombin
- Recombinant Proteins