Article
Expression, isolation, and characterization of an active site (serine 528----alanine) mutant of recombinant bovine prothrombin.
The Journal of biological chemistry - 25 May 1991
Pei G, Baker K, Emfinger S M, Fowlkes D M, Lentz B R
Abstract excerpt
An active site mutant bovine prothrombin cDNA (Ser528----Ala) has been constructed, subcloned, and expressed in Chinese hamster ovary cells. The recombinant mutant prothrombin, expressed at the level of 1.5-2.0 micrograms/ml of cell medium, was fully carboxylated (9.9 +/- 0.4 mol of gamma-carboxyglutamic acid/mol of prothrombin). The mutant prothrombin could be activated to thrombin by Taipan snake venom and...
Topics
- 1-Carboxyglutamic Acid
- Alanine
- Animals
- Binding Sites
- Cattle
- Chromatography, Liquid
- DNA
- Electrophoresis, Polyacrylamide Gel
- Endopeptidases
- Enzyme Activation
- Gene Expression Regulation