Article
Isolation and quantification of class 1 MHC gene mutants in mouse T cells by immunoselection with a magnetic cell sorter (MACS).
Journal of immunological methods - 2 May 1994
Wixler V, Klarmann B, Begemann U, Gahn G, Lorenz R, Hempel K
Abstract excerpt
A method is described for determining the frequency of cells with a mutation in an autosomal gene coding for a membrane protein. Using a monoclonal antibody to H-2Kk surface antigen and magnetic cell separation (MACS) more than 10(4)-fold enrichment of the H-2Kk negative population was achieved, as tested with artificial mixtures containing a known number of antigen-negative cells. After a second magnetic sorting...
Topics
- Animals
- Antibodies, Monoclonal
- Cell Division
- Cell Separation
- Cell Survival
- Erythrocytes
- Flow Cytometry
- Genes, MHC Class I
- H-2 Antigens
- Magnetics
- Mice