Article
Isolation of mouse Lyt2- mutant cells using magnetic particles.
Journal of immunological methods - 2 Oct 1992
Iannelli D, Capparelli R, Scala F, Ferrara L
Abstract excerpt
A procedure is described for the selection of mouse Lyt2- mutant cells. The procedure is based upon repeated cycles of selection with rat monoclonal antibodies to the Lyt2 antigen and magnetic particles coated with goat anti-mouse IgG. Stable Lyt2- mutant clones were derived from cells previously mutagenized with X rays and, at a lower frequency, also from non-mutagenized cells. The procedure can be used to...
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