Article
Rate and mechanism of generation of beta 2-microglobulin mutants from a heterozygous murine cell line.
Journal of immunology (Baltimore, Md. : 1950) - 1 Sept 1990
Rajan T V, Moffat L F, Frankel W N
Abstract excerpt
Mutants that fail to express the B2mb allele can be readily isolated from a B2ma/b heterozygous cell line called R8 by a single step immunoselection protocol using a mAb against beta 2 microglobulin protein in the presence of complement. We present data that demonstrate that the mutants preexist the selection and arise at a rate of about 10(-6)/cell per generation. Southern blot analyses suggest that the mutants...
Topics
- Animals
- Blotting, Southern
- Cell Line
- Chromosome Deletion
- Genes
- Genetic Linkage
- Heterozygote
- Karyotyping
- Mice
- Mutation
- Restriction Mapping
- beta 2-Microglobulin