Detection of DNA sequence variation via deliberate heteroduplex formation from genomic DNAs amplified en masse in "population tubes".

doi:10.1101/gr.3.4.225

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Article

Detection of DNA sequence variation via deliberate heteroduplex formation from genomic DNAs amplified en masse in "population tubes".

PCR methods and applications - 1 Feb 1994

Ruano G, Deinard A S, Tishkoff S, Kidd K K

Abstract excerpt

We have developed the population tube (poptube) system for sensitive detection and large-scale sampling of DNA sequence variation in several human populations of wide geographic distribution. In this methodology, genomic DNAs from five individuals in a population are PCR amplified en masse to max...

Topics

Animals
Base Sequence
DNA
Female
Genetic Variation
Glucosephosphate Dehydrogenase
Humans
Male
Molecular Sequence Data
Nucleic Acid Heteroduplexes
Pan troglodytes
Polymerase Chain Reaction

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