Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Detection of DNA sequence variation via deliberate heteroduplex formation from genomic DNAs amplified en masse in "population tubes".

PCR methods and applications - 1 Feb 1994

Ruano G, Deinard A S, Tishkoff S, Kidd K K

Abstract excerpt

We have developed the population tube (poptube) system for sensitive detection and large-scale sampling of DNA sequence variation in several human populations of wide geographic distribution. In this methodology, genomic DNAs from five individuals in a population are PCR amplified en masse to maximize deliberately the chances of forming heteroduplexes among allelic variants. Interpopulation mixing is performed in...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Base Sequence
Share to DNA
Share to Genetic Variation

Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

Share to Glucosephosphate Dehydrogenase

Share to Molecular Sequence Data

Share to Nucleic Acid Heteroduplexes

Share to Pan troglodytes

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 8173512
DOI: 10.1101/gr.3.4.225

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence. Graph snapshot: 2026-09-29.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Detection of DNA sequence variation via deliberate heteroduplex formation from genomic DNAs amplified en masse in "population tubes".PMID 8173512Read ↗

Minimizing carry-over PCR contamination in expanded CAG/CTG repeat instability applicationsSemantic proximity Read ↗

Detecting mitochondrial genotypes by temperature gradient gel electrophoresis and heteroduplex analysis.Semantic proximity Read ↗

Heteroduplex formation: a potential source of genotyping error from PCR products.Semantic proximity Read ↗

In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.Semantic proximity Read ↗

Heteroduplex analysis of minisatellite variability.Semantic proximity Read ↗

Detecting single base substitutions as heteroduplex polymorphismsSemantic proximity Read ↗

Rapid genotyping of CYP2D6, CYP2C19 and TPMT polymorphisms by primer extension reaction in a dipstick format.Semantic proximity Read ↗

Genetic screening and testing by induced heteroduplex formation.Semantic proximity Read ↗

Homozygous variegate porphyria: identification of mutations on both alleles of the protoporphyrinogen oxidase gene in a severely affected proband.Semantic proximity Read ↗

Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis.Semantic proximity Read ↗

Optimisation and properties of a UHG for genotyping of hemoglobins S and C.Semantic proximity Read ↗

Intramolecular controls for the generation of clinical-quality SNP genotypes and the assessment of normal heterozygote imbalance.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.