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Article

Detection of hemi/homozygotes through heteroduplex formation in high-resolution melting analysis.

Analytical biochemistry - 1 Mar 2011

Cheng Jinting, Yim Onn-Siong, Low Poh-Sim, Tay Stacey K H, Yap Eric P H, Lai Poh-San

Abstract excerpt

Heteroduplex formation, required for the complete detection of hemi/homozygotes using high-resolution melting analysis, can be induced either by pre-PCR mixing of genomic DNAs or by post-PCR mixing of PCR products from unknown and reference samples. This study investigates the effects of both methods using two single nucleotide polymorphisms in X-linked DMD gene. The results show that both methods resulted in the...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 21111703
DOI: 10.1016/j.ab.2010.11.031

Genes

DMD

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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