Article
Heteroduplex formation: a potential source of genotyping error from PCR products.
Prenatal diagnosis - 1 Mar 1993
Hatcher S L, Lambert Q T, Teplitz R L, Carlson J R
Abstract excerpt
The formation of heteroduplexes from polymerase chain reaction (PCR) products has recently become a diagnostic tool that is routinely used for the prenatal detection of small deletions or insertions in a number of disease-causing alleles. We present evidence illustrating that heterozygous PCR pro...
Topics
- Amniocentesis
- Base Sequence
- Chromosome Deletion
- DNA
- Diagnostic Errors
- Electrophoresis, Polyacrylamide Gel
- Female
- Fetal Diseases
- Genetic Carrier Screening
- Genotype
- Globins
- Homozygote
- Humans
- Male
- Molecular Sequence Data
- Nucleic Acid Heteroduplexes
- Polymerase Chain Reaction
- Pregnancy