Article
A simple and rapid technique for the detection of rifampin resistance in Mycobacterium leprae.
International journal of leprosy and other mycobacterial diseases : official organ of the International Leprosy Association - 1 Dec 1993
Honoré N, Perrani E, Telenti A, Grosset J, Cole S T
Abstract excerpt
The rifampin resistance of Mycobacterium leprae is due to missense mutations in the rpoB gene encoding the beta-subunit of the essential enzyme RNA polymerase. A rapid and very simple method has been developed to detect rifampin resistance in small numbers of M. leprae present in biopsies. It inv...
Topics
- Alleles
- Amino Acid Sequence
- Base Sequence
- DNA Primers
- DNA, Bacterial
- DNA, Single-Stranded
- DNA-Directed RNA Polymerases
- Dapsone
- Drug Resistance, Microbial
- Drug Therapy, Combination
- Genes, Bacterial
- Humans
- Leprosy, Lepromatous
- Male
- Molecular Sequence Data
- Mutation
- Mycobacterium leprae
- Polymerase Chain Reaction