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Detection of Rifampin-Resistant <i>Mycobacterium tuberculosis</i> in Sputa by Nested PCR-Linked Single-Strand Conformation Polymorphism and DNA Sequencing

1 Jul 2001

Abstract excerpt

Either PCR-mediated single strand conformation polymorphism (SSCP) analysis or DNA sequencing of rpoB DNA (157 bp) can be used as a rapid screening method for the detection of mutations related to the rifampin resistance of Mycobacterium tuberculosis. However, due to the nonspecific amplification of rpoB DNA from nontuberculous mycobacteria these methods cannot be directly applied to clinical specimens such as...

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PMID: 11427578
DOI: 10.1128/jcm.39.7.2610-2617.2001

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