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Article

Low-stringency single-specific-primer PCR as a tool for detection of mutations in the rpoB gene of rifampin-resistant Mycobacterium tuberculosis.

Journal of clinical microbiology - 1 Jul 2003

Carvalho Wania S, Spindola de Miranda Silvana, Costa Kátia M, Araújo José G V C, Augusto Claudio J, Pesquero João B, Pesquero Jorge L, Gomes Maria A

Abstract excerpt

By the low-stringency single-specific-primer PCR technique, a highly sensitive and rapid method for diagnosis of rifampin resistance in Mycobacterium tuberculosis was established. Seven rifampin-resistant and five rifampin-susceptible specimens were analyzed. Rifampin resistance was determined by MIC measurement. A complex electrophoretic pattern consisting of many bands was obtained for both susceptible and...

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Share to Antibiotics, Antitubercular
Share to DNA-Directed RNA Polymerases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Drug Resistance, Bacterial

Share to Electrophoresis, Polyacrylamide Gel

Share to Microbial Sensitivity Tests

Share to Mycobacterium tuberculosis

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Share to Tuberculosis, Pulmonary

Source

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Identifiers

PMID: 12843099
DOI: 10.1128/JCM.41.7.3384-3386.2003
PMCID: PMC165332

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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