Article
A yeast Ubc9 mutant protein with temperature-sensitive in vivo function is subject to conditional proteolysis by a ubiquitin- and proteasome-dependent pathway.
The Journal of biological chemistry - 18 Oct 1996
Betting J, Seufert W
Abstract excerpt
The UBC9 gene of the yeast Saccharomyces cerevisiae is essential for cell viability and encodes a soluble protein of the nucleus that is metabolically stable. Products of mutant alleles selected to confer temperature-sensitive in vivo function were found to be extremely short-lived at the restrictive but long-lived at the permissive condition. An extragenic suppressor mutation was isolated which increased...
Topics
- Alleles
- Anaphase-Promoting Complex-Cyclosome
- CDC28 Protein Kinase, S cerevisiae
- Cysteine Endopeptidases
- DNA Primers
- DNA, Bacterial
- Ligases
- Multienzyme Complexes
- Proteasome Endopeptidase Complex
- Saccharomyces cerevisiae
- Structure-Activity Relationship
- Suppression, Genetic
- Temperature