Article
Role of glycosylation in transport and enzymic activity of neutral endopeptidase-24.11.
The Biochemical journal - 1 Sept 1994
Lafrance M H, Vézina C, Wang Q, Boileau G, Crine P, Lemay G
Abstract excerpt
Neutral endopeptidase (NEP, EC 3.4.24.11) is a major ectoenzyme of the brush-border membrane. The ectodomain of NEP contains five putative N-glycosylation sites. In order to determine the role of the addition of sugar moieties on the activity and intracellular transport of NEP, we have used site-directed mutagenesis to remove all or some of the five potential sites of sugar addition in membrane-bound and secreted...
Topics
- Animals
- Biological Transport
- Cell Line
- Cell Membrane
- Computer Simulation
- DNA
- Electrophoresis, Polyacrylamide Gel
- Endoplasmic Reticulum
- Glycosylation
- Golgi Apparatus
- Immunoblotting