Article
Role of glycosylation in the renal electrogenic Na+-HCO3- cotransporter (NBCe1).
American journal of physiology. Renal physiology - 1 Jun 2003
Choi Inyeong, Hu Lihui, Rojas José D, Schmitt Bernhard M, Boron Walter F
Abstract excerpt
The electrogenic Na(+)-HCO(3)(-) cotransporter NBCe1 is important for the regulation of intracellular pH (pH(i)) and for epithelial HCO(3)(-) transport in many tissues, including kidney, pancreas, and brain. In the present study, we investigate glycosylation sites in NBCe1. Treatment of rat kidney membrane extracts with peptide N-glycosidase F (PNGase F) shifted the apparent molecular weight (MW) of NBCe1 from...
Topics
- Amidohydrolases
- Amino Acid Substitution
- Animals
- Cell Membrane
- Glycosylation
- Histocytochemistry
- Hydrogen-Ion Concentration
- Immunoblotting
- Kidney
- Lectins
- Microelectrodes
- Molecular Weight
- Mutagenesis, Site-Directed
- Mutation