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Article

Effects of site-directed removal of N-glycosylation sites in human erythropoietin on its production and biological properties.

The Journal of biological chemistry - 25 Oct 1991

Yamaguchi K, Akai K, Kawanishi G, Ueda M, Masuda S, Sasaki R

Abstract excerpt

Erythropoietin (Epo) has three N-linked sugar chains. Codons for asparagine at N-glycosylation sites in genomic human Epo DNA were replaced with those for glutamine. The wild-type Epo gene and seven mutants that lacked N-glycosylation sites in every possible combination were introduced into baby hamster-kidney cells. To study the role of the N-linked sugars in Epo biosynthesis, Epo protein expressed transiently...

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PMID: 1657925

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