Article
Mutation specific PCR and direct solid phase sequencing assay for the detection of hepatitis B virus pre-C/C mutants in anti-HBe-positive, chronic hepatitis B.
Journal of medical virology - 1 May 1994
Goergen B, Meyer zum Büschenfeld K H, Gerken G
Abstract excerpt
Sequence analysis of the HBV DNA from patients with anti-HBe+, chronic hepatitis B revealed that the lack of HBeAg is mostly due to a single G-->A transition at nucleotide position 1896, resulting in a translational stop codon. A point mutation-specific polymerase chain reaction (msPCR) for the d...
Topics
- Base Sequence
- DNA Primers
- DNA, Viral
- Genetic Variation
- Hepatitis B
- Hepatitis B Antibodies
- Hepatitis B e Antigens
- Hepatitis B virus
- Humans
- Molecular Sequence Data
- Point Mutation
- Polymerase Chain Reaction